EsturgeonV1, France, Analysis, bibRecord, 000100

Complex mutation at a microsatellite locus in sturgeons: Acipenser sinensis, A. schrenckii, A. gueldenstaedtii and A. baerii

Identifieur interne : 000100 ( France/Analysis ); précédent : 000099; suivant : 000101

Complex mutation at a microsatellite locus in sturgeons: Acipenser sinensis, A. schrenckii, A. gueldenstaedtii and A. baerii

Auteurs : Z. Shao [République populaire de Chine] ; S. Lek [France] ; J. Chang [République populaire de Chine]

Source :

Journal of Applied Ichthyology [ 0175-8659 ] ; 2005-02.

RBID : ISTEX:268866F25457D1A62D0D6F76FEF9CF7D39C8D036

Abstract

Cross‐species amplifications of microsatellite locus Spl‐106, which was originally screened from the genome of shovelnose sturgeon (Scaphirhynchus platorynchus) with a perfect TAGA repeat motif, were carried out in four other species of the genera Acipenser. A total of 34 polymerase chain reaction (PCR) products representing 16 different alleles of this locus was sequenced. Sequence analysis results showed that besides the number changes of repeat units, many mutational events, such as single‐base substitutions and various insertion/deletion (indels) occurred not only at species level but also at individual level, even among the different alleles within the same individual. The repeat motifs varied from perfect (TAGA)n array to perfect compound (TAAA)m (GAAA)n and perfect or imperfect compound (TAAA)m (TAGA)n (TAAA)x arrays in different species and different individuals. The evolution dynamics of this locus in sturgeons was inferred in that it may evolve from a single perfect to different perfect or imperfect compounds.

Url:

https://api.istex.fr/document/268866F25457D1A62D0D6F76FEF9CF7D39C8D036/fulltext/pdf

DOI: 10.1111/j.1439-0426.2004.00629.x

Affiliations:

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ISTEX:268866F25457D1A62D0D6F76FEF9CF7D39C8D036

Le document en format XML

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<author><name sortKey="Chang, J" sort="Chang, J" uniqKey="Chang J" first="J." last="Chang">J. Chang</name>
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<front><div type="abstract" xml:lang="en">Cross‐species amplifications of microsatellite locus Spl‐106, which was originally screened from the genome of shovelnose sturgeon (Scaphirhynchus platorynchus) with a perfect TAGA repeat motif, were carried out in four other species of the genera Acipenser. A total of 34 polymerase chain reaction (PCR) products representing 16 different alleles of this locus was sequenced. Sequence analysis results showed that besides the number changes of repeat units, many mutational events, such as single‐base substitutions and various insertion/deletion (indels) occurred not only at species level but also at individual level, even among the different alleles within the same individual. The repeat motifs varied from perfect (TAGA)n array to perfect compound (TAAA)m (GAAA)n and perfect or imperfect compound (TAAA)m (TAGA)n (TAAA)x arrays in different species and different individuals. The evolution dynamics of this locus in sturgeons was inferred in that it may evolve from a single perfect to different perfect or imperfect compounds.</div>
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Complex mutation at a microsatellite locus in sturgeons: Acipenser sinensis, A. schrenckii, A. gueldenstaedtii and A. baerii

Complex mutation at a microsatellite locus in sturgeons: Acipenser sinensis, A. schrenckii, A. gueldenstaedtii and A. baerii

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri